CO inhibits cell death and caspase activation induced by Fas activating antibody Jo2. MLEC cultures were pretreated in the absence or presence of CO (250 ppm) for 2 h prior to the addition of antibody Jo2 (200 ng/ml) for the indicated times. 200 μl of supernatant medium was removed for LDH assays as described in Methods (A). The data represent an average of two independent experiments with each sample in triplicate (n = 3). Data from Jo2 treated cells in the presence of CO were compared with control (Jo2 treatment alone) cells at each time point using Student's T-test (*P < 0.05). The total lysates were subjected to Western blot to detect caspase-9 and caspase-3. β-actin served as the standard (B). Westerns are representative of three independent experiments.
Wang et al. Medical Gas Research 2011 1:8 doi:10.1186/2045-9912-1-8